Inhalt des Dokuments
Molekulares Screening, Isolierung und Charakterisierung von konjugativen Plasmiden aus kontaminierten Böden
Objectives and individual tasks of the joint project:Work carried out in India:
- Screening for both conjugative plasmids from gram positive bacteria and gram negative bacteria from highly contaminated soil by the triparental mating method.
- Isolation and incompatibility group/oriT-family-based characterization of conjugative plasmids from gram positive and gram negative bacteria from differently contaminated soils from various origins.
Work carried out in Germany:
- Design of a gfp-labelled pIP501-based mobilizable plasmid to capture conjugative plasmids from differently contaminated Indian and German soil samples.
- Establishment of a biofilm reactor system with bacterial isolates from different soil samples and plasmid transfer experiments with gfp-labelled pIP501 derivative
- Establishment of biofilm reactors with contaminated soil as carrier material from both India and Germany and plasmid transfer experiments with gfp-labelled pIP501 derivative
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