direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Page Content

List of Publications of the Chair

Adequacy of in situ glass slides and direct sand extractions to assess the microbiota within sand columns used for drinking water treatment.
Citation key Laangmark2001
Author J. Långmark and N. J. Ashbolt and U. Szewzyk and T. A. Stenström
Pages 601–607
Year 2001
DOI 10.1139/w01-052
Journal Canadian Journal of Microbiology
Volume 47
Number 7
Month Jul
Institution Swedish Institute for Infectious Disease Control, Solna.
Abstract Historically, Cholodny-Rossi buried glass slide techniques have been used to study the microbiota of subsurface environments, yet the bias of such a technique has not been compared against direct sand extraction using modern in situ probing. Over a period of 34 wk, four separate 4-m-deep sand columns receiving raw lake water were examined to compare direct extraction of sand filter biofilm material against in situ glass slide biofilms. Significantly different DAPI direct counts and fluorescent in situ hybridization signals for major phylogenetic groups were observed. Not only were lower proportions (P < 0.001) of EUB338-probed DAPI cells observed on in situ glass slides, but also fewer gamma-Proteobacteria (12\%-21\%) and more alpha-Proteobacteria (16\%-33\%) when compared to direct sand extracts. Hence, investigators of the microbial ecology of even simple sand biofilms must consider the inherent biases from "accepted" methods and seek further independent methods to identify those which may be most accurate.
Link to publication Download Bibtex entry

Zusatzinformationen / Extras

Quick Access:

Schnellnavigation zur Seite über Nummerneingabe

Head of Chair

Prof. Dr. rer. nat. Ulrich Szewzyk
sec. BH 6-1
Room BH-N 604
Tel +49 30 314 73 461


Bärbel Minx
sec. BH 6-1
Room BH-N 603
Tel +49 30 314 73 460

Consultation hours:
Mon - Fri

Adress Chair of Environmental Microbiology

Environmental Microbiology
Institute of Environmental Technology
Faculty Faculty of Process Sciences
sec. BH 6-1
Ernst-Reuter-Platz 1
10587 Berlin
Tel +49 30 314 73 460
Fax +49 30 314 73 673