direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Page Content

List of Publications of the Chair

In situ visualization of glycoside hydrolase family 92 genes in marine flavobacteria
Citation key Zeugner2021
Author Zeugner, L. E. and Krüger, K. and Barrero-Canosa, J. and Amann, R. I. and Fuchs, B. M.
Year 2021
DOI https://doi.org/10.1038/s43705-021-00082-4
Journal ISME Communications 1
Volume 2021
Number 81
Month December
Abstract Gene clusters rich in carbohydrate-active enzymes within Flavobacteriia genera provide a competitiveness for their hosts to degrade diatom-derived polysaccharides. One such widely distributed polysaccharide is glucuronomannan, a main cell wall component of diatoms. A conserved gene cluster putatively degrading glucuronomannan was found previously among various flavobacterial taxa in marine metagenomes. Here, we aimed to visualize two glycoside hydrolase family 92 genes coding for α-mannosidases with fluorescently-labeled polynucleotide probes using direct-geneFISH. Reliable in situ localization of single-copy genes was achieved with an efficiency up to 74% not only in the flavobacterial strains Polaribacter Hel1_33_49 and Formosa Hel1_33_131 but also in planktonic samples from the North Sea. In combination with high-resolution microscopy, direct-geneFISH gave visual evidence of the contrasting lifestyles of closely related Polaribacter species in those samples and allowed for the determination of gene distribution among attached and free-living cells. We also detected highly similar GH92 genes in yet unidentified taxa by broadening probe specificities, enabling a visualization of the functional trait in subpopulations across the borders of species and genera. Such a quantitative insight into the niche separation of flavobacterial taxa complements our understanding of the ecology of polysaccharide-degrading bacteria beyond omics-based techniques on a single-cell level.
Download Bibtex entry

Zusatzinformationen / Extras

Quick Access:

Schnellnavigation zur Seite über Nummerneingabe

Head of Chair

Prof. Dr. rer. nat. Ulrich Szewzyk
BH 6-1
BH-N 604
Tel +49 30 314 73 461

Secretariat

Bärbel Minx
BH 6-1
BH-N 603
Tel +49 30 314 73 460

Consultation hours:
Mon - Fri

Adress Chair of Environmental Microbiology

Environmental Microbiology
of Environmental Technology
Faculty of Process Sciences
BH 6-1
Ernst-Reuter-Platz 1
10587 Berlin
Tel +49 30 314 73 460
Fax +49 30 314 73 673