direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Inhalt des Dokuments

Publikationsliste des Fachgebiets

Tigecycline resistance in clinical isolates of Enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(L) and tet(M)
Zitatschlüssel Fiedler20150
Autor Fiedler, S. and Bender, J.K. and Klare, I. and Halbedel, S. and Grohmann, E. and Szewzyk, U. and Werner, G.
Jahr 2015
DOI 10.1093/jac/dkv420
Journal J. Antimicrob. Chemother.
Monat December
Zusammenfassung OBJECTIVES: Tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including VRE and MRSA. The German National Reference Centre for Staphylococci and Enterococci has received 73 tigecycline-resistant Enterococcus faecium and Enterococcus faecalis isolates in recent years. The precise mechanism of how enterococci become resistant to tigecycline remains undetermined. This study documents an analysis of the role of efflux pumps in tigecycline resistance in clinical isolates of Enterococcus spp. METHODS: Various tigecycline MICs were found for the different isolates analysed. Tigecycline-resistant strains were analysed with respect to genome and transcriptome differences by means of WGS and RT-qPCR. Genes of interest were cloned and expressed in Listeria monocytogenes for verification of their functionality. RESULTS: Detailed comparative whole-genome analyses of three isogenic strains, showing different levels of tigecycline resistance, revealed the major facilitator superfamily (MFS) efflux pump TetL and the ribosomal protection protein TetM as possible drug resistance proteins. Subsequent RT-qPCR confirmed up-regulation of the respective genes. A correlation of gene copy number and level of MIC was inferred from further qPCR analyses. Expression of both tet(L) and tet(M) in L. monocytogenes unequivocally demonstrated the potential to increase tigecycline MICs upon acquisition of either locus. CONCLUSIONS: Our results indicate that increased expression of two tetracycline resistance determinants, a tet(L)-encoded MFS pump and a tet(M)-encoded ribosomal protection protein, is capable of conferring tigecycline resistance in enterococcal clinical isolates.
Download Bibtex Eintrag

Zusatzinformationen / Extras


Schnellnavigation zur Seite über Nummerneingabe


Prof. Dr. rer. nat. Ulrich Szewzyk
Raum BH-N 604
Tel +49 30 314 73 461
Fax +49 30 314 73 673

Mi 12:00 - 13:00 Uhr

Administrative Fragen?

Melden Sie sich bitte
zuerst im Sekretariat

Sekretariat UMB

Bärbel Minx
Sekr. BH 6-1
Raum BH-N 603
Tel +49 30 314 73 460

Adresse Fachgebiet Umweltmikrobiologie

Technische Universität Berlin
Institut für technischen Umweltschutz
Fakultät III
Sekr. BH 6-1
Ernst-Reuter-Platz 1
10587 Berlin
Tel +49 30 314 73 460
Fax +49 30 314 73 673