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Detection of conjugative plasmids and antibiotic resistance genes in anthropogenic soils from Germany and India.
Zitatschlüssel Malik2008
Autor Malik, A. and Celik, E.-K. and Bohn, C. and Böckelmann, U. and Knobel, K. and Grohmann, E.
Seiten 207–216
Jahr 2008
DOI 10.1111/j.1574-6968.2007.01030.x
Journal FEMS Microbiology Letters
Jahrgang 279
Nummer 2
Monat Feb
Institution Technische Universität Berlin, FG Umweltmikrobiologie/Genetik, Franklinstrasse 28/29, Berlin, Germany.
Zusammenfassung PCR typing methods were used to assess the presence of plasmids of the incompatibility (Inc) groups IncP, IncN, IncW, IncQ and rolling circle plasmids of the pMV158 type in total DNA extracts from anthropogenic soils from India and Germany. Ten different soils from two different locations in Germany, the urban park Berlin Tiergarten and the abandoned sewage field Berlin-Buch, and from four different locations in India were analysed. PCR amplification of the total DNA extracts revealed the prevalence of IncP-specific sequences in Berlin Buch and Indian soil samples. The detected IncP plasmids contained at least one transfer function, the origin of transfer, oriT. In contrast to IncP-specific sequences, IncQ, IncN, IncW and pMV158-specific sequences were never detected. The presence of ampC, tet (O), ermB, SHV-5, mecA, and vanA antibiotic resistance genes was also tested. Three Indian soil samples irrigated with wastewater contained the ampC gene, whereas the other resistance genes were not found in any of the samples. Detection of IncP trfA2 and oriT sequences by PCR amplification and hybridization is a clear indication that IncP plasmids are prevalent in these habitats. Exogenous plasmid isolation revealed conjugative plasmids belonging to the IncPbeta group encoding resistance to ampicillin.
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