TU Berlin

Environmental MicrobiologyDAAD PPP

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Intracellular Localization of T4SLS proteins in Enterococci

Fluorescence micrograph: Localization of a transfer protein in Enterococcus faecalis (pIP501)
Lupe

Our aim is to elucidate the role of the protein components in the assembly of a conjugative T4S-like machinery in Gram-positive bacteria. We will verify our in vivo and in vitro protein-protein interaction studies and computer predictions by localization of the T4SLS proteins in Enterococcus faecalis cell compartments by cell fractionation followed by Western Blot, immunogold transmission electron microscopy and immunofluorescence microscopy.

Molecular detection and imaging will give us important knowledge on the sub-cellular localization of the T4SL-system. Targeted interruption of DNA transfer based on detailed structure/function knowledge of T4SLS-systems, could reduce the spread of resistance and virulence genes and help treat bacterial infections in the future.
The project is a collaboration between the Department of Microbiology and Virology, the Department of Electron Microscopy, University of Tromsø, Norway, Technische Universität Berlin, Germany, and Karl-Franzens Universität Graz, Austria.

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